› 4hpb

X-ray diffraction
1.6Å resolution

Crystal structure of Nitrophorin 4 from Rhodnius prolixus Complexed with Beta-Mercaptoethanol at pH 7.4

Released:
DOI: 10.2210/pdb4hpb/pdb
PDB entry 4hpb coloured by chain, front view.
PDB entry 4hpb coloured by chain, side view.
PDB entry 4hpb coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Complexes of ferriheme nitrophorin 4 with low-molecular weight thiol(ate)s occurring in blood plasma.
He C, Nishikawa K, Erdem ÖF, Reijerse E, Ogata H, Lubitz W, Knipp M
J Inorg Biochem 122 38-48 (2013)
PMID: 23474537

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 70.288Å b: 43.185Å c: 52.618Å
α: 90° β: 94.38° γ: 90°
R-values:
R R work R free
0.144 0.142 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Venoms of Heteropteran Insects: A Treasure Trove of Diverse Pharmacological Toolkits.
Walker et al. (2016)
3 other articles

1 mention without citation

Structural analysis of Cytochrome P450 BM3 mutant M11 in complex with dithiothreitol.
Frydenvang et al. (2019)