› 4hg4

X-ray diffraction
3.2Å resolution

Crystal structure of Fab 2G1 in complex with a H2N2 influenza virus hemagglutinin

Released:
DOI: 10.2210/pdb4hg4/pdb
PDB entry 4hg4 coloured by chain, front view.
PDB entry 4hg4 coloured by chain, side view.
PDB entry 4hg4 coloured by chain, top view.
Source organisms:
Primary publication:
A recurring motif for antibody recognition of the receptor-binding site of influenza hemagglutinin.
Xu R, Krause JC, McBride R, Paulson JC, Crowe JE, Wilson IA
Nat Struct Mol Biol 20 363-70 (2013)
PMID: 23396351

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208955 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin HA1 chain Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 327 amino acids
Theoretical weight: 36.57 KDa
Source organism: Influenza A virus (A/Japan/305+/1957(H2N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03451 (Residues: 15-340; Coverage: 59%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: a, b, c, d, e, f, g, h, i
Molecule details ›
Chains: a, b, c, d, e, f, g, h, i
Length: 174 amino acids
Theoretical weight: 20.14 KDa
Source organism: Influenza A virus (A/Japan/305+/1957(H2N2))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P03451 (Residues: 341-514; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Fab 2G1 heavy chain Chains: J, L, N, P, R, T, V, X, Z
Molecule details ›
Chains: J, L, N, P, R, T, V, X, Z
Length: 223 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Fab 2G1 light chain Chains: K, M, O, Q, S, U, W, Y, z
Molecule details ›
Chains: K, M, O, Q, S, U, W, Y, z
Length: 214 amino acids
Theoretical weight: 23.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 126.842Å b: 133.143Å c: 812.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.249 0.302
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

25 review citations

Continuing challenges in influenza.
Webster et al. (2014)
24 more

11 mentions without citation

Structural characterization of viral epitopes recognized by broadly cross-reactive antibodies.
Lee et al. (2015)
10 more