› 4hdi

X-ray diffraction
2.45Å resolution

Crystal Structure of 3E5 IgG3 FAB from mus musculus

Released:
DOI: 10.2210/pdb4hdi/pdb
PDB entry 4hdi coloured by chain, front view.
PDB entry 4hdi coloured by chain, side view.
PDB entry 4hdi coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Global structures of IgG isotypes expressing identical variable regions.
Eryilmaz E, Janda A, Kim J, Cordero RJ, Cowburn D, Casadevall A
Mol Immunol 56 588-98 (2013)
PMID: 23911417

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106877 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chains: A, L
Molecule details ›
Chains: A, L
Length: 219 amino acids
Theoretical weight: 24.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A2NHM3 (Residues: 1-219; Coverage: 100%)
Gene names: Igk-C, Igkc
Sequence domains:
Structure domains: Immunoglobulins
Ig heavy chain Mem5 Chains: B, H
Molecule details ›
Chains: B, H
Length: 220 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P84751 (Residues: 1-106, 107-220; Coverage: 92%)
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 67.07Å b: 98.289Å c: 142.363Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.208 0.263
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Signaling by Antibodies: Recent Progress.
Bournazos et al. (2017)
3 more

6 mentions without citation

Antibody-Mediated Catalysis in Infection and Immunity.
Bowen et al. (2017)
5 more