› 4h8r

X-ray diffraction
1.25Å resolution

Imipenem complex of GES-5 carbapenemase

Released:
DOI: 10.2210/pdb4h8r/pdb
PDB entry 4h8r coloured by chain, front view.
PDB entry 4h8r coloured by chain, side view.
PDB entry 4h8r coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural basis for progression toward the carbapenemase activity in the GES family of β-lactamases.
Smith CA, Frase H, Toth M, Kumarasiri M, Wiafe K, Munoz J, Mobashery S, Vakulenko SB
J Am Chem Soc 134 19512-5 (2012)
PMID: 23148776

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase GES-5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.22 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09HD0 (Residues: 1-287; Coverage: 100%)
Gene names: blaGES-5, pGES-GZ_027
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand IM2 2 x IM2
Ligand IOD 17 x IOD
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 42.94Å b: 81.24Å c: 71.93Å
α: 90° β: 102.04° γ: 90°
R-values:
R R work R free
0.149 0.148 0.17
Expression system: Escherichia coli

Quick links

Citations

2 review citations

β-Lactamases and β-Lactamase Inhibitors in the 21st Century.
Tooke et al. (2019)
1 more

5 mentions without citation

Structural basis for carbapenem-hydrolyzing mechanisms of carbapenemases conferring antibiotic resistance.
Jeon et al. (2015)
4 more