› 4h6u

X-ray diffraction
2.45Å resolution

Tubulin acetyltransferase mutant

Released:
DOI: 10.2210/pdb4h6u/pdb
PDB entry 4h6u coloured by chain, front view.
PDB entry 4h6u coloured by chain, side view.
PDB entry 4h6u coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Crystal structures of tubulin acetyltransferase reveal a conserved catalytic core and the plasticity of the essential N terminus.
Kormendi V, Szyk A, Piszczek G, Roll-Mecak A
J Biol Chem 287 41569-75 (2012)
PMID: 23105108

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [alpha-tubulin]-L-lysine = CoA + [alpha-tubulin]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-tubulin N-acetyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.35 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6PH17 (Residues: 1-188; Coverage: 63%)
Gene names: atat1, mec17, si:ch211-152p11.5, zgc:65893
Sequence domains: GNAT acetyltransferase, Mec-17
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand ACO 2 x ACO
2 bound ligands:
Ligand K 1 x K
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P43212
Unit cell:
a: 58.128Å b: 58.128Å c: 225.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.248
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Tubulin acetylation: responsible enzymes, biological functions and human diseases.
Li et al. (2015)
6 more

1 mention without citation

Crystal structures of tubulin acetyltransferase reveal a conserved catalytic core and the plasticity of the essential N terminus.
Kormendi et al. (2012)