4h62

X-ray diffraction
3Å resolution

Structure of the Saccharomyces cerevisiae Mediator subcomplex Med17C/Med11C/Med22C

Released:
Primary publication:
Structure of the Mediator head module.
Nature 492 448-51 (2012)
PMID: 23123849

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-152366 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Mediator of RNA polymerase II transcription subunit 11 Chain: K
Molecule details ›
Chain: K
Length: 40 amino acids
Theoretical weight: 4.34 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99278 (Residues: 84-115; Coverage: 28%)
Gene names: MED11, YM9718.11C, YMR112C
Mediator of RNA polymerase II transcription subunit 22 Chain: V
Molecule details ›
Chain: V
Length: 31 amino acids
Theoretical weight: 3.4 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32570 (Residues: 96-121; Coverage: 22%)
Gene names: MED22, SRB6, YBR1721, YBR253W
Mediator of RNA polymerase II transcription subunit 17 Chain: Q
Molecule details ›
Chain: Q
Length: 312 amino acids
Theoretical weight: 36.55 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32569 (Residues: 377-687; Coverage: 45%)
Gene names: MED17, SRB4, YER022W
Sequence domains: Subunit 17 of Mediator complex

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 261.23Å b: 261.23Å c: 47.74Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.228
Expression system: Escherichia coli