› 4gf7

X-ray diffraction
1.58Å resolution

Crystal structure of 2-Methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (MHPCO), unliganded form

Released:
DOI: 10.2210/pdb4gf7/pdb
PDB entry 4gf7 coloured by chain, front view.
PDB entry 4gf7 coloured by chain, side view.
PDB entry 4gf7 coloured by chain, top view.
Source organism: Mesorhizobium japonicum MAFF 303099
Entry authors: Kobayashi J, Yoshida H, Mikami B, Hayashi H, Kamitori S, Sawa Y, Yagi T

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 379 amino acids
Theoretical weight: 41.8 KDa
Source organism: Mesorhizobium japonicum MAFF 303099
Expression system: Escherichia coli
UniProt:
  • Canonical: Q988D3 (Residues: 1-379; Coverage: 100%)
Gene name: mlr6788
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand BME 1 x BME
Ligand GOL 5 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I222
Unit cell:
a: 49.363Å b: 131.287Å c: 132.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.192
Expression system: Escherichia coli

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