› 4gct

X-ray diffraction
2.45Å resolution

structure of No factor protein-DNA complex

Released:
DOI: 10.2210/pdb4gct/pdb
PDB entry 4gct coloured by chain, front view.
PDB entry 4gct coloured by chain, side view.
PDB entry 4gct coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA
Proc Natl Acad Sci U S A 110 10586-91 (2013)
PMID: 23754405

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117540 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nucleoid occlusion factor SlmA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 196 amino acids
Theoretical weight: 22.81 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KVD2 (Residues: 1-196; Coverage: 100%)
Gene names: VC_0214, slmA
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
DNA (5'-D(*TP*TP*AP*CP*GP*TP*GP*AP*GP*TP*AP*CP*TP*CP*AP*CP*GP*TP*AP*A)-3') Chains: W, Z
Molecule details ›
Chains: W, Z
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 69.1Å b: 61.36Å c: 121.08Å
α: 90° β: 99.06° γ: 90°
R-values:
R R work R free
0.225 0.225 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Subcellular Organization: A Critical Feature of Bacterial Cell Replication.
Surovtsev et al. (2018)
16 more

6 mentions without citation

SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.
Tonthat et al. (2013)
5 more