› 4gai

X-ray diffraction
1.49Å resolution

Crystal structure of EBI-005, a chimera of human IL-1beta and IL-1Ra

Released:
DOI: 10.2210/pdb4gai/pdb
PDB entry 4gai coloured by chain, front view.
PDB entry 4gai coloured by chain, side view.
PDB entry 4gai coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design of a superior cytokine antagonist for topical ophthalmic use.
Hou J, Townson SA, Kovalchin JT, Masci A, Kiner O, Shu Y, King BM, Schirmer E, Golden K, Thomas C, Garcia KC, Zarbis-Papastoitsis G, Furfine ES, Barnes TM
Proc Natl Acad Sci U S A 110 3913-8 (2013)
PMID: 23431173

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interleukin-1 beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 153 amino acids
Theoretical weight: 17.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01584 (Residues: 117-255, 256-269; Coverage: 57%)
Gene names: IL1B, IL1F2
Sequence domains: Interleukin-1 / 18
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 43.392Å b: 66.91Å c: 216.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.219
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structural Basis of IL-1 Family Cytokine Signaling.
Fields et al. (2019)
11 more

5 mentions without citation

Design of a superior cytokine antagonist for topical ophthalmic use.
Hou et al. (2013)
4 more