› 4g3g

X-ray diffraction
2.5Å resolution

Crystal structure of murine NF-kappaB inducing kinase (NIK) V408L bound to a 2-(aminothiazolyl)phenol (cmp3)

Released:
DOI: 10.2210/pdb4g3g/pdb
PDB entry 4g3g coloured by chain, front view.
PDB entry 4g3g coloured by chain, side view.
PDB entry 4g3g coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The crystal structure of the catalytic domain of the NF-κB inducing kinase reveals a narrow but flexible active site.
de Leon-Boenig G, Bowman KK, Feng JA, Crawford T, Everett C, Franke Y, Oh A, Stanley M, Staben ST, Starovasnik MA, Wallweber HJ, Wu J, Wu LC, Johnson AR, Hymowitz SG
Structure 20 1704-14 (2012)
PMID: 22921830

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 14 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 38.81 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9WUL6 (Residues: 345-675; Coverage: 35%)
Gene names: Map3k14, Nik
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 0WA 1 x 0WA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2221
Unit cell:
a: 74.423Å b: 135.32Å c: 64.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.196 0.252
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

The non-canonical NF-κB pathway in immunity and inflammation.
Sun SC. (2017)
10 more

2 mentions without citation

Pharmacological inhibition of NF-κB-inducing kinase (NIK) with small molecules for the treatment of human diseases.
Cheng et al. (2021)
1 more