› 4fzv

X-ray diffraction
2Å resolution

Crystal structure of the human MTERF4:NSUN4:SAM ternary complex

Released:
DOI: 10.2210/pdb4fzv/pdb
PDB entry 4fzv coloured by chain, front view.
PDB entry 4fzv coloured by chain, side view.
PDB entry 4fzv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification.
Yakubovskaya E, Guja KE, Mejia E, Castano S, Hambardjieva E, Choi WS, Garcia-Diaz M
Structure 20 1940-7 (2012)
PMID: 23022348

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182298 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
5-methylcytosine rRNA methyltransferase NSUN4 Chain: A
Molecule details ›
Chain: A
Length: 359 amino acids
Theoretical weight: 40.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CB9 (Residues: 26-384; Coverage: 94%)
Gene name: NSUN4
Sequence domains: 16S rRNA methyltransferase RsmB/F
Structure domains:
mTERF domain-containing protein 2 processed Chain: B
Molecule details ›
Chain: B
Length: 239 amino acids
Theoretical weight: 27.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z6M4 (Residues: 122-330; Coverage: 55%)
Gene names: HSPC096, MTERF4, MTERFD2
Sequence domains: mTERF

Ligands and Environments

3 bound ligands:
Ligand SAM 1 x SAM
Ligand FMT 1 x FMT
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 298.667Å b: 53.226Å c: 52.963Å
α: 90° β: 98.75° γ: 90°
R-values:
R R work R free
0.166 0.165 0.19
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Maintenance and Expression of Mammalian Mitochondrial DNA.
Gustafsson et al. (2016)
15 more

6 mentions without citation

Structure of the essential MTERF4:NSUN4 protein complex reveals how an MTERF protein collaborates to facilitate rRNA modification.
Yakubovskaya et al. (2012)
5 more