› 4fru

X-ray diffraction
1.1Å resolution

Crystal structure of horse wild-type cyclophilin B

Released:
DOI: 10.2210/pdb4fru/pdb
PDB entry 4fru coloured by chain, front view.
PDB entry 4fru coloured by chain, side view.
PDB entry 4fru coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Crystal structures of wild-type and mutated cyclophilin B that causes hyperelastosis cutis in the American quarter horse.
Boudko SP, Ishikawa Y, Lerch TF, Nix J, Chapman MS, Bächinger HP
BMC Res Notes 5 626 (2012)
PMID: 23137129

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107782 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-prolyl cis-trans isomerase Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.47 KDa
Source organism: Equus caballus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5YBL8 (Residues: 34-216; Coverage: 85%)
Gene name: PPIB
Sequence domains: Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Structure domains: Cyclophilin-like

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ME2 1 x ME2
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 64.88Å b: 44.07Å c: 60.57Å
α: 90° β: 95.2° γ: 90°
R-values:
R R work R free
0.117 0.116 0.139
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Molecular insights into prolyl and lysyl hydroxylation of fibrillar collagens in health and disease.
Gjaltema et al. (2017)
2 other articles

6 mentions without citation

Hemolytic lectin CEL-III heptamerizes via a large structural transition from α-helices to a β-barrel during the transmembrane pore formation process.
Unno et al. (2014)
5 more