› 4fna

X-ray diffraction
3.5Å resolution

Structure of unliganded FhuD2 from Staphylococcus Aureus

Released:
DOI: 10.2210/pdb4fna/pdb
PDB entry 4fna coloured by chain, front view.
PDB entry 4fna coloured by chain, side view.
PDB entry 4fna coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal and solution structure analysis of FhuD2 from Staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-B.
Podkowa KJ, Briere LA, Heinrichs DE, Shilton BH
Biochemistry 53 2017-31 (2014)
PMID: 24606332

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 280 amino acids
Theoretical weight: 31.62 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7BGA5 (Residues: 25-302; Coverage: 98%)
Gene names: A6760_08565, BN1321_380120, E1948_02835, E1948_12070, EP54_10225, EQ90_01480, FAF17_01170, G6W63_00485, G6Y24_09080, GO814_07590, GO942_01330, GQX37_08105, GZ156_00990, GZ163_04885, H2639_07850, HMPREF3211_01612, M1K003_0826, NCTC10702_03592, NCTC13131_01627, SAMEA1531744_00395, SAMEA2080344_00518, SAMEA2081063_01511, SAMEA4008575_00998, SAMEA70146418_00450, SAMEA70245418_00502, fhuD, fhuD2, fhud2
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: I422
Unit cell:
a: 207.507Å b: 207.507Å c: 162.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Recent developments in understanding the iron acquisition strategies of gram positive pathogens.
Sheldon et al. (2015)
6 more

3 mentions without citation

Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development.
Marchetti et al. (2020)
2 more