4ff4

X-ray diffraction
2.03Å resolution

N4 mini-vRNAP transcription initiation complex, 4 min after soaking GTP, ATP and Mn

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-117478 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Virion DNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1118 amino acids
Theoretical weight: 123.19 KDa
Source organism: Escherichia virus N4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q859P9 (Residues: 998-2103; Coverage: 32%)
Gene name: 50
Sequence domains:
Structure domains:
Bacteriophage N4 P2 promoter Chains: C, D
Molecule details ›
Chains: C, D
Length: 36 nucleotides
Theoretical weight: 11.02 KDa
Source organism: Enquatrovirus N4
Expression system: Not provided

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 82.398Å b: 111.779Å c: 276.894Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.191 0.239
Expression systems:
  • Escherichia coli
  • Not provided