› 4f40

X-ray diffraction
1.6Å resolution

X-ray crystal structure of Apo prostaglandin f synthase from Leishmania major Friedlin

Released:
DOI: 10.2210/pdb4f40/pdb
PDB entry 4f40 coloured by chain, front view.
PDB entry 4f40 coloured by chain, side view.
PDB entry 4f40 coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structures of prostaglandin F synthase from the protozoa Leishmania major and Trypanosoma cruzi with NADP.
Moen SO, Fairman JW, Barnes SR, Sullivan A, Nakazawa-Hewitt S, Van Voorhis WC, Staker BL, Lorimer DD, Myler PJ, Edwards TE
Acta Crystallogr F Struct Biol Commun 71 609-14 (2015)
PMID: 25945716

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149509 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
9,11-endoperoxide prostaglandin H2 reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 288 amino acids
Theoretical weight: 32.19 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: P22045 (Residues: 1-284; Coverage: 100%)
Gene name: P100/11E
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

3 bound ligands:
Ligand CIT 2 x CIT
Ligand EDO 18 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 94.55Å b: 34.59Å c: 107.2Å
α: 90° β: 103.22° γ: 90°
R-values:
R R work R free
0.155 0.154 0.18
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Leishmania braziliensis prostaglandin F2α synthase impacts host infection.
Alves-Ferreira et al. (2020)
3 more

3 mentions without citation

Protease inhibitors broadly effective against feline, ferret and mink coronaviruses.
Perera et al. (2018)
2 more