› 4f32

X-ray diffraction
1.9Å resolution

Function and Biology Details

Reaction catalysed: 
A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107368 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-oxoacyl-[acyl-carrier-protein] synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 451 amino acids
Theoretical weight: 46.8 KDa
Source organism: Burkholderia vietnamiensis G4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4JL30 (Residues: 1-430; Coverage: 100%)
Gene name: Bcep1808_4002
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1
Unknown peptide Chain: C
Molecule details ›
Chain: C
Length: 3 amino acids
Theoretical weight: 273 Da
Source organism: Streptomyces platensis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand N32 2 x N32
Ligand EDO 2 x EDO
1 modified residue:
Ligand CSU 2 x CSU

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 55.49Å b: 100.84Å c: 143.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.17
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

The design and analysis of transposon insertion sequencing experiments.
Chao et al. (2016)
10 more

3 mentions without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)
2 more