› 4f01

X-ray diffraction
1.4Å resolution

Crystal structure of an artificial dimeric DnaK complex

Released:
DOI: 10.2210/pdb4f01/pdb
PDB entry 4f01 coloured by chain, front view.
PDB entry 4f01 coloured by chain, side view.
PDB entry 4f01 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural studies on the forward and reverse binding modes of peptides to the chaperone DnaK.
Zahn M, Berthold N, Kieslich B, Knappe D, Hoffmann R, Sträter N
J Mol Biol 425 2463-79 (2013)
PMID: 23562829

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-141446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein DnaK Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 26.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6Y8 (Residues: 389-607; Coverage: 34%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 60.11Å b: 61.38Å c: 69.19Å
α: 90° β: 103.39° γ: 90°
R-values:
R R work R free
0.163 0.162 0.213
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Hsp70 chaperone dynamics and molecular mechanism.
Mayer MP. (2013)
11 more

6 mentions without citation

De novo transcriptome sequencing and analysis of the cereal cyst nematode, Heterodera avenae.
Kumar et al. (2014)
5 more