› 4ex9

X-ray diffraction
3.15Å resolution

Crystal structure of the prealnumycin C-glycosynthase AlnA in complex with ribulose 5-phosphate

Released:
DOI: 10.2210/pdb4ex9/pdb
PDB entry 4ex9 coloured by chain, front view.
PDB entry 4ex9 coloured by chain, side view.
PDB entry 4ex9 coloured by chain, top view.
Source organism: Streptomyces sp. CM020
Primary publication:
Structural basis for C-ribosylation in the alnumycin A biosynthetic pathway.
Oja T, Niiranen L, Sandalova T, Klika KD, Niemi J, Mäntsälä P, Schneider G, Metsä-Ketelä M
Proc Natl Acad Sci U S A 110 1291-6 (2013)
PMID: 23297194

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AlnA Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 32.95 KDa
Source organism: Streptomyces sp. CM020
Expression system: Escherichia coli
UniProt:
  • Canonical: B6SEG5 (Residues: 2-306; Coverage: 100%)
Gene name: alnA
Sequence domains: Indigoidine synthase A like protein
Structure domains: Indigoidine synthase domain

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand 5RP 1 x 5RP
Ligand EPE 1 x EPE
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I4132
Unit cell:
a: 168.79Å b: 168.79Å c: 168.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.248
Expression system: Escherichia coli

Quick links

Citations

7 review citations

A comprehensive review of glycosylated bacterial natural products.
Elshahawi et al. (2015)
6 more

1 mention without citation

Structural basis for C-ribosylation in the alnumycin A biosynthetic pathway.
Oja et al. (2013)