› 4ep7

X-ray diffraction
2.28Å resolution

Functional implications from the Cid1 poly(U) polymerase crystal structure

Released:
DOI: 10.2210/pdb4ep7/pdb
PDB entry 4ep7 coloured by chain, front view.
PDB entry 4ep7 coloured by chain, side view.
PDB entry 4ep7 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Functional implications from the Cid1 poly(U) polymerase crystal structure.
Munoz-Tello P, Gabus C, Thore S
Structure 20 977-86 (2012)
PMID: 22608966

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase cid1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 38.97 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O13833 (Residues: 40-377; Coverage: 84%)
Gene names: SPAC19D5.03, cid1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand UTP 2 x UTP
Ligand MG 7 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 53.49Å b: 76.86Å c: 81.86Å
α: 90° β: 91.17° γ: 90°
R-values:
R R work R free
0.191 0.188 0.247
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Cytoplasmic RNA: a case of the tail wagging the dog.
Norbury CJ. (2013)
7 more

2 mentions without citation

Structural plasticity of Cid1 provides a basis for its distributive RNA terminal uridylyl transferase activity.
Yates et al. (2015)
1 more