› 4ekf

X-ray diffraction
0.98Å resolution

Structure of the Inactive Adenovirus Proteinase at 0.98 Angstrom Resolution

Released:
DOI: 10.2210/pdb4ekf/pdb
PDB entry 4ekf coloured by chain, front view.
PDB entry 4ekf coloured by chain, side view.
PDB entry 4ekf coloured by chain, top view.
Source organism: Human adenovirus 2
Primary publication:
Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: III. atomic resolution structure of the nascent form of the adenovirus proteinase.
Baniecki ML, McGrath WJ, Mangel WF
J Biol Chem 288 2081-91 (2013)
PMID: 23043139

Function and Biology Details

Reaction catalysed: 
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136669 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 23.15 KDa
Source organism: Human adenovirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03252 (Residues: 1-204; Coverage: 100%)
Gene name: L3
Sequence domains: Adenovirus endoprotease
Structure domains: Adenoviral Proteinase; Chain A

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 36.27Å b: 54.54Å c: 42.41Å
α: 90° β: 100.1° γ: 90°
R-values:
R R work R free
0.135 not available 0.168
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure, function and dynamics in adenovirus maturation.
Mangel et al. (2014)
3 more

4 mentions without citation

Structure and Function of Viral Deubiquitinating Enzymes.
Bailey-Elkin et al. (2017)
3 more