› 4e9d

X-ray diffraction
2.75Å resolution

The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with 3-(1-benzothiophen-2-yl)propanoyl-derivatized DPPLHSpTA peptide

Released:
DOI: 10.2210/pdb4e9d/pdb
PDB entry 4e9d coloured by chain, front view.
PDB entry 4e9d coloured by chain, side view.
PDB entry 4e9d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-throughput interrogation of ligand binding mode using a fluorescence-based assay.
Śledź P, Lang S, Stubbs CJ, Abell C
Angew Chem Int Ed Engl 51 7680-3 (2012)
PMID: 22730171

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156768 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 371-594; Coverage: 37%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
3-(1-benzothiophen-2-yl)propanoyl-derivatized DPPLHSpTA peptide Chain: E
Molecule details ›
Chain: E
Length: 10 amino acids
Theoretical weight: 1.1 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P1
Unit cell:
a: 35.873Å b: 36.302Å c: 47.474Å
α: 85.84° β: 77.21° γ: 68.24°
R-values:
R R work R free
0.27 0.267 0.33
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Recent Advances and New Strategies in Targeting Plk1 for Anticancer Therapy.
Lee et al. (2015)
1 more

2 mentions without citation

Identification of potential Plk1 targets in a cell-cycle specific proteome through structural dynamics of kinase and Polo box-mediated interactions.
Bibi et al. (2013)
1 more