› 4e67

X-ray diffraction
2.1Å resolution

The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with hydrocinnamoyl-derivatized PLHSpTA peptide

Released:
DOI: 10.2210/pdb4e67/pdb
PDB entry 4e67 coloured by chain, front view.
PDB entry 4e67 coloured by chain, side view.
PDB entry 4e67 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Using ligand-mapping simulations to design a ligand selectively targeting a cryptic surface pocket of polo-like kinase 1.
Tan YS, Śledź P, Lang S, Stubbs CJ, Spring DR, Abell C, Best RB
Angew Chem Int Ed Engl 51 10078-81 (2012)
PMID: 22961729

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156766 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 26.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P53350 (Residues: 371-594; Coverage: 37%)
Gene names: PLK, PLK1
Sequence domains: POLO box duplicated region
Structure domains: POLO box domain
hydrocinnamoyl-derivatized PLHSpTA peptide Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 836 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 35.492Å b: 50.906Å c: 57.91Å
α: 90° β: 101.01° γ: 90°
R-values:
R R work R free
0.218 0.216 0.265
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Recent Advances and New Strategies in Targeting Plk1 for Anticancer Therapy.
Lee et al. (2015)
10 more

3 mentions without citation

Using ligand-mapping simulations to design a ligand selectively targeting a cryptic surface pocket of polo-like kinase 1.
Tan et al. (2012)
2 more