› 4e2u

X-ray diffraction
1.58Å resolution

Crystal Structures of RadAmin intein from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb4e2u/pdb
PDB entry 4e2u coloured by chain, front view.
PDB entry 4e2u coloured by chain, side view.
PDB entry 4e2u coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein.
Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwaï H
J Mol Biol 421 85-99 (2012)
PMID: 22560994

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pho RadA intein Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 19.13 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58001 (Residues: 150-326; Coverage: 32%)
Gene names: PH0263, radA
Sequence domains: Intein splicing domain
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 46.93Å b: 63.649Å c: 66.552Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.195
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Protein splicing: how inteins escape from precursor proteins.
Mills et al. (2014)
4 more

4 mentions without citation

Structural and dynamical features of inteins and implications on protein splicing.
Eryilmaz et al. (2014)
3 more