› 4e19

X-ray diffraction
1.41Å resolution

Crystal structure of RNase H1 from halophilic archaeon Halobacterium salinarum NRC-1

Released:
DOI: 10.2210/pdb4e19/pdb
PDB entry 4e19 coloured by chain, front view.
PDB entry 4e19 coloured by chain, side view.
PDB entry 4e19 coloured by chain, top view.
Source organism: Halobacterium salinarum NRC-1
Entry authors: You DJ, Angkawidjaja C, Koga Y, Kanaya S

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HI Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 21 KDa
Source organism: Halobacterium salinarum NRC-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HSF6 (Residues: 1-199; Coverage: 100%)
Gene names: VNG_0255C, rnhA
Sequence domains: Reverse transcriptase-like
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand MN 5 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I41
Unit cell:
a: 115.802Å b: 115.802Å c: 38.282Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.207
Expression system: Escherichia coli

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