› 4dzo

X-ray diffraction
1.76Å resolution

Structure of Human Mad1 C-terminal Domain Reveals Its Involvement in Kinetochore Targeting

Released:
DOI: 10.2210/pdb4dzo/pdb
PDB entry 4dzo coloured by chain, front view.
PDB entry 4dzo coloured by chain, side view.
PDB entry 4dzo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting.
Kim S, Sun H, Tomchick DR, Yu H, Luo X
Proc Natl Acad Sci U S A 109 6549-54 (2012)
PMID: 22493223

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic spindle assembly checkpoint protein MAD1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y6D9 (Residues: 597-718; Coverage: 17%)
Gene names: MAD1, MAD1L1, TXBP181
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 44.767Å b: 44.767Å c: 211.056Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.16 0.197
Expression system: Escherichia coli BL21(DE3)

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Citations

13 review citations

The spindle assembly checkpoint.
Lara-Gonzalez et al. (2012)
12 more

9 mentions without citation

MAD1: Kinetochore Receptors and Catalytic Mechanisms.
Luo et al. (2018)
8 more