› 4dn5

X-ray diffraction
2.5Å resolution

Crystal Structure of NF-kB-inducing Kinase (NIK)

Released:
DOI: 10.2210/pdb4dn5/pdb
PDB entry 4dn5 coloured by chain, front view.
PDB entry 4dn5 coloured by chain, side view.
PDB entry 4dn5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the nuclear factor κB-inducing kinase (NIK) kinase domain reveals a constitutively active conformation.
Liu J, Sudom A, Min X, Cao Z, Gao X, Ayres M, Lee F, Cao P, Johnstone S, Plotnikova O, Walker N, Chen G, Wang Z
J Biol Chem 287 27326-34 (2012)
PMID: 22718757

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase kinase kinase 14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 39.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q99558 (Residues: 330-680; Coverage: 37%)
Gene names: MAP3K14, NIK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand GOL 1 x GOL
Ligand EDO 6 x EDO
Ligand AGS 2 x AGS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41
Unit cell:
a: 85.06Å b: 85.06Å c: 115.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.222
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

The non-canonical NF-κB pathway in immunity and inflammation.
Sun SC. (2017)
9 more

8 mentions without citation

The structural pathway of interleukin 1 (IL-1) initiated signaling reveals mechanisms of oncogenic mutations and SNPs in inflammation and cancer.
Acuner Ozbabacan et al. (2014)
7 more