› 4dmw

X-ray diffraction
2.5Å resolution

Crystal structure of the GT domain of Clostridium difficile toxin A (TcdA) in complex with UDP and Manganese

Released:
DOI: 10.2210/pdb4dmw/pdb
PDB entry 4dmw coloured by chain, front view.
PDB entry 4dmw coloured by chain, side view.
PDB entry 4dmw coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
The structure of Clostridium difficile toxin A glucosyltransferase domain bound to Mn2+ and UDP provides insights into glucosyltransferase activity and product release.
D'Urzo N, Malito E, Biancucci M, Bottomley MJ, Maione D, Scarselli M, Martinelli M
FEBS J 279 3085-97 (2012)
PMID: 22747490

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcdA Chain: A
Molecule details ›
Chain: A
Length: 556 amino acids
Theoretical weight: 64.54 KDa
Source organism: Clostridioides difficile
Expression system: Brevibacillus choshinensis
UniProt:
  • Canonical: P16154 (Residues: 1-541; Coverage: 20%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 66.66Å b: 156.73Å c: 65.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.259
Expression system: Brevibacillus choshinensis

Quick links

Citations

3 review citations

The role of toxins in Clostridium difficile infection.
Chandrasekaran et al. (2017)
2 more

2 mentions without citation

Biophysical Characterization and Activity of Lymphostatin, a Multifunctional Virulence Factor of Attaching and Effacing Escherichia coli.
Cassady-Cain et al. (2016)
1 more