› 4dkw

X-ray diffraction
2.02Å resolution

Structure of P22 Large terminase nuclease domain

Released:
DOI: 10.2210/pdb4dkw/pdb
PDB entry 4dkw coloured by chain, front view.
PDB entry 4dkw coloured by chain, side view.
PDB entry 4dkw coloured by chain, top view.
Source organism: Salmonella virus P22
Primary publication:
Structure of p22 headful packaging nuclease.
Roy A, Cingolani G
J Biol Chem 287 28196-205 (2012)
PMID: 22715098

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminase, large subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 211 amino acids
Theoretical weight: 24.61 KDa
Source organism: Salmonella virus P22
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26745 (Residues: 289-499; Coverage: 42%)
Gene name: 2
Sequence domains: Terminase RNaseH-like domain
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 60.921Å b: 139.795Å c: 61.005Å
α: 90° β: 95.1° γ: 90°
R-values:
R R work R free
0.167 0.166 0.197
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Molecular architecture of tailed double-stranded DNA phages.
Fokine et al. (2014)
7 more

5 mentions without citation

The structure of the herpes simplex virus DNA-packaging terminase pUL15 nuclease domain suggests an evolutionary lineage among eukaryotic and prokaryotic viruses.
Selvarajan Sigamani et al. (2013)
4 more