› 4dkk

X-ray diffraction
1.7Å resolution

The X-ray Crystal Structure of the Human STAU1 SSM-'RBD'5 Domain-Swapped Dimer

Released:
DOI: 10.2210/pdb4dkk/pdb
PDB entry 4dkk coloured by chain, front view.
PDB entry 4dkk coloured by chain, side view.
PDB entry 4dkk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay.
Gleghorn ML, Gong C, Kielkopf CL, Maquat LE
Nat Struct Mol Biol 20 515-24 (2013)
PMID: 23524536

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Double-stranded RNA-binding protein Staufen homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95793 (Residues: 448-557; Coverage: 19%)
Gene names: STAU, STAU1
Sequence domains: Staufen C-terminal domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FLC 1 x FLC
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P41212
Unit cell:
a: 45.741Å b: 45.741Å c: 86.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.163 0.202
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Retrotransposons as regulators of gene expression.
Elbarbary et al. (2016)
8 more

10 mentions without citation

'Black sheep' that don't leave the double-stranded RNA-binding domain fold.
Gleghorn et al. (2014)
9 more