› 4d2j

X-ray diffraction
1.75Å resolution

Crystal structure of F16BP Aldolase from Toxoplasma gondii (TgALD1)

Released:
DOI: 10.2210/pdb4d2j/pdb
PDB entry 4d2j coloured by chain, front view.
PDB entry 4d2j coloured by chain, side view.
PDB entry 4d2j coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Structural and functional divergence of the aldolase fold in Toxoplasma gondii.
Tonkin ML, Halavaty AS, Ramaswamy R, Ruan J, Igarashi M, Ngô HM, Boulanger MJ
J Mol Biol 427 840-852 (2015)
PMID: 25284756

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-bisphosphate aldolase Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 37.82 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B9PW35 (Residues: 77-422; Coverage: 80%)
Gene names: BN1205_061250, TGVEG_236040
Sequence domains: Fructose-bisphosphate aldolase class-I
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand BME 1 x BME
Ligand SO4 2 x SO4
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P42212
Unit cell:
a: 109.814Å b: 109.814Å c: 54.357Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.231
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Protein acetylation in the critical biological processes in protozoan parasites.
Maran et al. (2021)
5 other articles

2 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
1 more