› 4czm

X-ray diffraction
2.2Å resolution

C. crescentus MreB, monomeric, AMPPNP

Released:
DOI: 10.2210/pdb4czm/pdb
PDB entry 4czm coloured by chain, front view.
PDB entry 4czm coloured by chain, side view.
PDB entry 4czm coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
Bacterial actin MreB forms antiparallel double filaments.
van den Ent F, Izoré T, Bharat TA, Johnson CM, Löwe J
Elife 3 e02634 (2014)
PMID: 24843005

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell shape-determining protein MreB Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 36.89 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3C7V4 (Residues: 9-347; Coverage: 98%)
Gene names: CCNA_01612, mreB
Sequence domains: MreB/Mbl protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P43212
Unit cell:
a: 67.791Å b: 67.791Å c: 320.556Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Bacterial morphogenesis and the enigmatic MreB helix.
Errington J. (2015)
26 more

8 mentions without citation

Bacterial actin MreB forms antiparallel double filaments.
van den Ent et al. (2014)
7 more