› 4czf

X-ray diffraction
1.64Å resolution

C. crescentus MreB, single filament, ADP

Released:
DOI: 10.2210/pdb4czf/pdb
PDB entry 4czf coloured by chain, front view.
PDB entry 4czf coloured by chain, side view.
PDB entry 4czf coloured by chain, top view.
Source organism: Caulobacter vibrioides
Primary publication:
Bacterial actin MreB forms antiparallel double filaments.
van den Ent F, Izoré T, Bharat TA, Johnson CM, Löwe J
Elife 3 e02634 (2014)
PMID: 24843005

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell shape-determining protein MreB Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 36.87 KDa
Source organism: Caulobacter vibrioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3C7V4 (Residues: 9-347; Coverage: 98%)
Gene names: CCNA_01612, mreB
Sequence domains: MreB/Mbl protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P21
Unit cell:
a: 51.548Å b: 71.815Å c: 53.731Å
α: 90° β: 100.37° γ: 90°
R-values:
R R work R free
0.174 0.172 0.196
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Bacterial morphogenesis and the enigmatic MreB helix.
Errington J. (2015)
26 more

4 mentions without citation

Bacterial actin MreB forms antiparallel double filaments.
van den Ent et al. (2014)
3 more