› 4cbw

X-ray diffraction
2.5Å resolution

Crystal structure of Plasmodium berghei actin I with D-loop from muscle actin

Released:
DOI: 10.2210/pdb4cbw/pdb
PDB entry 4cbw coloured by chain, front view.
PDB entry 4cbw coloured by chain, side view.
PDB entry 4cbw coloured by chain, top view.
Source organisms:
Primary publication:
Structural differences explain diverse functions of Plasmodium actins.
Vahokoski J, Bhargav SP, Desfosses A, Andreadaki M, Kumpula EP, Martinez SM, Ignatev A, Lepper S, Frischknecht F, Sidén-Kiamos I, Sachse C, Kursula I
PLoS Pathog 10 e1004091 (2014)
PMID: 24743229

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146436 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle; Actin-1 Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 41.94 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q4Z1L3 (Residues: 2-39, 77-100, 104-376; Coverage: 89%)
  • Canonical: P68137 (Residues: 41-77, 103-104; Coverage: 10%)
Gene names: ACT1, ACTA, ACTA1, ACTI, PB000323.01.0, PBANKA_1459300
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 127 amino acids
Theoretical weight: 14.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13020 (Residues: 50-174; Coverage: 17%)
Gene names: Gsb, Gsn
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand DIO 1 x DIO
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P212121
Unit cell:
a: 54.24Å b: 69.53Å c: 178.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.264
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The Molecular Basis of Erythrocyte Invasion by Malaria Parasites.
Cowman et al. (2017)
7 more

4 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
3 more