› 4cbe

X-ray diffraction
1.83Å resolution

Crystal structure of complement factors H and FHL-1 binding protein BBH06 or CRASP-2 from Borrelia burgdorferi (Native)

Released:
DOI: 10.2210/pdb4cbe/pdb
PDB entry 4cbe coloured by chain, front view.
PDB entry 4cbe coloured by chain, side view.
PDB entry 4cbe coloured by chain, top view.
Source organism: Borreliella burgdorferi B31
Primary publication:
Structural characterization of CspZ, a complement regulator factor H and FHL-1 binding protein from Borrelia burgdorferi.
Brangulis K, Petrovskis I, Kazaks A, Bogans J, Otikovs M, Jaudzems K, Ranka R, Tars K
FEBS J 281 2613-22 (2014)
PMID: 24702793

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129334 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Complement regulator-acquiring surface protein 2 (CRASP-2) Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 25.05 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O50665 (Residues: 23-236; Coverage: 98%)
Gene names: BB_H06, cspZ
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21212
Unit cell:
a: 90.69Å b: 48.84Å c: 44.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Complement Evasion Contributes to Lyme Borreliae-Host Associations.
Lin et al. (2020)
2 more

3 mentions without citation

Interaction with the host: the role of fibronectin and extracellular matrix proteins in the adhesion of Gram-negative bacteria.
Vaca et al. (2020)
2 more