4bzg

X-ray diffraction
2.13Å resolution

Crystal structure of galactose mutarotase GalM from Bacillus subtilis in complex with maltose

Released:
Entry authors: Vanden Broeck A, Sauvage E, Herman R, Kerff F, Duez C, Charlier P

Function and Biology Details

Reaction catalysed:
Alpha-D-glucose = beta-D-glucose
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153964 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Aldose 1-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 37.14 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P39840 (Residues: 2-325; Coverage: 100%)
Gene names: BSU18360, galM, yoxA
Sequence domains: Aldose 1-epimerase
Structure domains: Beta-galactosidase; Chain A, domain 5

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 82.461Å b: 84.202Å c: 125.445Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.227
Expression system: Escherichia coli K-12