› 4bwp

X-ray diffraction
3.6Å resolution

Structure of Drosophila Melanogaster PAN3 pseudokinase

Released:
DOI: 10.2210/pdb4bwp/pdb
PDB entry 4bwp coloured by chain, front view.
PDB entry 4bwp coloured by chain, side view.
PDB entry 4bwp coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure of the PAN3 pseudokinase reveals the basis for interactions with the PAN2 deadenylase and the GW182 proteins.
Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E
Mol Cell 51 360-73 (2013)
PMID: 23932717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAN2-PAN3 deadenylation complex subunit PAN3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.99 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95RR8 (Residues: 349-790; Coverage: 56%)
Gene names: CG11486, PAN3
Sequence domains: Pan3 Pseudokinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AN2 2 x AN2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P4322
Unit cell:
a: 102.171Å b: 102.171Å c: 264.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

28 review citations

Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation.
O'Brien et al. (2018)
27 more

4 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
3 more