› 4bu0

X-ray diffraction
1.5Å resolution

Crystal structure of Rad4 BRCT1,2 in complex with a Crb2 phosphopeptide

Released:
DOI: 10.2210/pdb4bu0/pdb
PDB entry 4bu0 coloured by chain, front view.
PDB entry 4bu0 coloured by chain, side view.
PDB entry 4bu0 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4(TopBP1).
Qu M, Rappas M, Wardlaw CP, Garcia V, Ren JY, Day M, Carr AM, Oliver AW, Du LL, Pearl LH
Mol Cell 51 723-736 (2013)
PMID: 24074952

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152286 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
S-M checkpoint control protein rad4 Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.24 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32372 (Residues: 1-186; Coverage: 29%)
Gene names: SPAC23C4.18c, cut5, rad4
Sequence domains:
Structure domains: BRCT domain
DNA repair protein crb2 Chains: B, C
Molecule details ›
Chains: B, C
Length: 16 amino acids
Theoretical weight: 1.84 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided
UniProt:
  • Canonical: P87074 (Residues: 180-193; Coverage: 2%)
Gene names: SPBC342.05, crb2, rhp9

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand ACT 1 x ACT
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21212
Unit cell:
a: 84.823Å b: 63.72Å c: 53.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.194
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

TopBP1: A BRCT-scaffold protein functioning in multiple cellular pathways.
Wardlaw et al. (2014)
2 more

4 mentions without citation

Phosphorylation-dependent assembly of DNA damage response systems and the central roles of TOPBP1.
Day et al. (2021)
3 more