› 4bph

X-ray diffraction
1.8Å resolution

High resolution crystal structure of Bacillus subtilis DltC

Released:
DOI: 10.2210/pdb4bph/pdb
PDB entry 4bph coloured by chain, front view.
PDB entry 4bph coloured by chain, side view.
PDB entry 4bph coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
High-resolution structures of the D-alanyl carrier protein (Dcp) DltC from Bacillus subtilis reveal equivalent conformations of apo- and holo-forms.
Zimmermann S, Pfennig S, Neumann P, Yonus H, Weininger U, Kovermann M, Balbach J, Stubbs MT
FEBS Lett 589 2283-9 (2015)
PMID: 26193422

Function and Biology Details

Reaction catalysed: 
ATP + D-alanine + poly(ribitol phosphate) = AMP + diphosphate + O-D-alanyl-poly(ribitol phosphate)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153896 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanyl carrier protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 10.09 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39579 (Residues: 1-78; Coverage: 100%)
Gene names: BSU38520, dltC, ipa-3r
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

2 bound ligands:
Ligand PNS 1 x PNS
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6522
Unit cell:
a: 49.207Å b: 49.207Å c: 146.264Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.167 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The many faces and important roles of protein-protein interactions during non-ribosomal peptide synthesis.
Izoré et al. (2018)
1 more

7 mentions without citation

Classification and energetics of the base-phosphate interactions in RNA.
Zirbel et al. (2009)
6 more