› 4bne

X-ray diffraction
2.57Å resolution

Pacsin2 Interacts with Membranes and Actin-Filaments

Released:
DOI: 10.2210/pdb4bne/pdb
PDB entry 4bne coloured by chain, front view.
PDB entry 4bne coloured by chain, side view.
PDB entry 4bne coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Direct interaction of actin filaments with F-BAR protein pacsin2.
Kostan J, Salzer U, Orlova A, Törö I, Hodnik V, Senju Y, Zou J, Schreiner C, Steiner J, Meriläinen J, Nikki M, Virtanen I, Carugo O, Rappsilber J, Lappalainen P, Lehto VP, Anderluh G, Egelman EH, Djinović-Carugo K
EMBO Rep 15 1154-62 (2014)
PMID: 25216944

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C and casein kinase substrate in neurons protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 457 amino acids
Theoretical weight: 52.91 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13154 (Residues: 1-448; Coverage: 100%)
Gene name: PACSIN2
Sequence domains:
Structure domains: Arfaptin homology (AH) domain/BAR domain

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 101.06Å b: 105.5Å c: 125.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Caveolae: One Function or Many?
Cheng et al. (2016)
12 more

2 mentions without citation

Direct interaction of actin filaments with F-BAR protein pacsin2.
Kostan et al. (2014)
1 more