› 4blk

X-ray diffraction
1.05Å resolution

Crystal Structure of Fungal Versatile Peroxidase I from Pleurotus ostreatus - Crystal Form I

Released:
DOI: 10.2210/pdb4blk/pdb
PDB entry 4blk coloured by chain, front view.
PDB entry 4blk coloured by chain, side view.
PDB entry 4blk coloured by chain, top view.
Source organism: Pleurotus ostreatus
Primary publication:
Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.
Fernández-Fueyo E, Ruiz-Dueñas FJ, Martínez MJ, Romero A, Hammel KE, Medrano FJ, Martínez AT
Biotechnol Biofuels 7 2 (2014)
PMID: 24387130

Function and Biology Details

Reaction catalysed: 
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H(2)O(2) = 4-hydroxy-3-methoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194079 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Versatile peroxidase VPL1 Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 34.79 KDa
Source organism: Pleurotus ostreatus
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: Q9UR19 (Residues: 31-361; Coverage: 98%)
Gene name: vpl1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS
Spacegroup: P43
Unit cell:
a: 96.5Å b: 96.5Å c: 38.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.137
Expression system: Escherichia coli str. K-12 substr. W3110

Quick links

Citations

9 review citations

Lignin-degrading enzymes.
Pollegioni et al. (2015)
8 more

3 mentions without citation

Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability.
Fernández-Fueyo et al. (2014)
2 more