› 4baj

X-ray diffraction
2.3Å resolution

MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser

Released:
DOI: 10.2210/pdb4baj/pdb
PDB entry 4baj coloured by chain, front view.
PDB entry 4baj coloured by chain, side view.
PDB entry 4baj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.
Pereira PJ, Royant A, Panjikar S, de Sanctis D
J Struct Biol 181 89-94 (2013)
PMID: 23178456

Function and Biology Details

Reaction catalysed: 
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chorismate synthase Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 43.19 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPY1 (Residues: 1-401; Coverage: 100%)
Gene names: MTCY159.16, Rv2540c, aroC, aroF
Sequence domains: Chorismate synthase
Structure domains: Chorismate synthase AroC

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: OXFORD DIFFRACTION GEMINI
Spacegroup: P6422
Unit cell:
a: 131.82Å b: 131.82Å c: 160.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.19 0.209
Expression system: Escherichia coli

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