› 4b8b

X-ray diffraction
2.8Å resolution

N-Terminal domain of the yeast Not1

Released:
DOI: 10.2210/pdb4b8b/pdb
PDB entry 4b8b coloured by chain, front view.
PDB entry 4b8b coloured by chain, side view.
PDB entry 4b8b coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Architecture of the nuclease module of the yeast Ccr4-not complex: the Not1-Caf1-Ccr4 interaction.
Basquin J, Roudko VV, Rode M, Basquin C, Séraphin B, Conti E
Mol Cell 48 207-18 (2012)
PMID: 22959269

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General negative regulator of transcription subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 603 amino acids
Theoretical weight: 68.6 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25655 (Residues: 151-753; Coverage: 29%)
Gene names: CDC39, NOT1, ROS1, YCR093W, YCR1151, YCR93W
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand AU 29 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 55.097Å b: 164.507Å c: 86.141Å
α: 90° β: 100.05° γ: 90°
R-values:
R R work R free
0.24 0.238 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Towards a molecular understanding of microRNA-mediated gene silencing.
Jonas et al. (2015)
18 more

3 mentions without citation

Regulation of the multisubunit CCR4-NOT deadenylase in the initiation of mRNA degradation.
Raisch et al. (2022)
2 more