› 4atv

X-ray diffraction
3.5Å resolution

STRUCTURE OF A TRIPLE MUTANT OF THE NHAA DIMER, CRYSTALLISED AT LOW PH

Released:
DOI: 10.2210/pdb4atv/pdb
PDB entry 4atv coloured by chain, front view.
PDB entry 4atv coloured by chain, side view.
PDB entry 4atv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.
Lee C, Yashiro S, Dotson DL, Uzdavinys P, Iwata S, Sansom MS, von Ballmoos C, Beckstein O, Drew D, Cameron AD
J Gen Physiol 144 529-44 (2014)
PMID: 25422503

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146739 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) antiporter NhaA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 401 amino acids
Theoretical weight: 43.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P13738 (Residues: 1-388; Coverage: 100%)
Gene names: JW0018, ant, b0019, nhaA
Sequence domains: Na+/H+ antiporter 1
Structure domains: Na+/H+ antiporter like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand LMU 1 x LMU
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 115.76Å b: 99.4Å c: 140.23Å
α: 90° β: 97.35° γ: 90°
R-values:
R R work R free
0.288 0.287 0.313
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
7 more

10 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
9 more