› 4atq

X-ray diffraction
2.75Å resolution

GABA-transaminase A1R958 in complex with external aldimine PLP-GABA adduct

Released:
DOI: 10.2210/pdb4atq/pdb
PDB entry 4atq coloured by chain, front view.
PDB entry 4atq coloured by chain, side view.
PDB entry 4atq coloured by chain, top view.
Source organism: Paenarthrobacter aurescens TC1
Primary publication:
Structures of a γ-aminobutyrate (GABA) transaminase from the s-triazine-degrading organism Arthrobacter aurescens TC1 in complex with PLP and with its external aldimine PLP-GABA adduct.
Bruce H, Nguyen Tuan A, Mangas Sánchez J, Leese C, Hopwood J, Hyde R, Hart S, Turkenburg JP, Grogan G
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1175-80 (2012)
PMID: 23027742

Function and Biology Details

Reaction catalysed: 
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-aminobutyrate transaminase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 456 amino acids
Theoretical weight: 47.63 KDa
Source organism: Paenarthrobacter aurescens TC1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1R958 (Residues: 1-456; Coverage: 100%)
Gene names: AAur_3069, gabT
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 12 x PLP
1 bound ligand:
Ligand ABU 12 x ABU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21212
Unit cell:
a: 176.71Å b: 292.83Å c: 105.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Alteration of the Donor/Acceptor Spectrum of the (S)-Amine Transaminase from Vibrio fluvialis.
Genz et al. (2015)
4 more

4 mentions without citation

Metabolic engineering of Corynebacterium glutamicum for enhanced production of 5-aminovaleric acid.
Shin et al. (2016)
3 more