› 4ass

X-ray diffraction
7Å resolution

TubR bound to tubC - 26 bp - from Bacillus thuringiensis serovar israelensis pBtoxis

Released:
DOI: 10.2210/pdb4ass/pdb
PDB entry 4ass coloured by chain, front view.
PDB entry 4ass coloured by chain, side view.
PDB entry 4ass coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Superstructure of the centromeric complex of TubZRC plasmid partitioning systems.
Aylett CH, Löwe J
Proc Natl Acad Sci U S A 109 16522-7 (2012)
PMID: 23010931

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117220 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional repressor TubR Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 104 amino acids
Theoretical weight: 12.12 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8KNP2 (Residues: 1-104; Coverage: 100%)
Gene names: ATN07_33545, pBt157, tubR
TUBC FROM BACILLUS THURINGIENSIS PBTOXIS 26 BP Chain: Y
Molecule details ›
Chain: Y
Length: 26 nucleotides
Theoretical weight: 7.92 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided
TUBC FROM BACILLUS THURINGIENSIS PBTOXIS 26 BP Chain: Z
Molecule details ›
Chain: Z
Length: 26 nucleotides
Theoretical weight: 8.05 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 54 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I222
Unit cell:
a: 87.561Å b: 162.352Å c: 285.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.412 0.408 0.484
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Bacterial Filament Systems: Toward Understanding Their Emergent Behavior and Cellular Functions.
Eun et al. (2015)
3 more

2 mentions without citation

Superstructure of the centromeric complex of TubZRC plasmid partitioning systems.
Aylett et al. (2012)
1 more