› 4as4

X-ray diffraction
1.7Å resolution

Structure of human inositol monophosphatase 1

Released:
DOI: 10.2210/pdb4as4/pdb
PDB entry 4as4 coloured by chain, front view.
PDB entry 4as4 coloured by chain, side view.
PDB entry 4as4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens.
Singh N, Halliday AC, Knight M, Lack NA, Lowe E, Churchill GC
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 1149-52 (2012)
PMID: 23027737

Function and Biology Details

Reactions catalysed: 
Myo-inositol phosphate + H(2)O = myo-inositol + phosphate
Alpha-D-galactose 1-phosphate + H(2)O = D-galactose + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol monophosphatase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 277 amino acids
Theoretical weight: 30.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29218 (Residues: 1-277; Coverage: 100%)
Gene names: IMPA, IMPA1
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand MG 6 x MG
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 60.613Å b: 76.199Å c: 117.648Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.152 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Defective craniofacial development and brain function in a mouse model for depletion of intracellular inositol synthesis.
Ohnishi et al. (2014)
6 more

6 mentions without citation

Analysis of expressed sequence tag loci on wheat chromosome group 4.
Miftahudin et al. (2004)
5 more