› 4al3

X-ray diffraction
1.98Å resolution

peptide deformylase (Co-form) with mercaptoethanol

Released:
DOI: 10.2210/pdb4al3/pdb
PDB entry 4al3 coloured by chain, front view.
PDB entry 4al3 coloured by chain, side view.
PDB entry 4al3 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
A FRET enzyme-based probe for monitoring hydrogen sulfide.
Strianese M, Palm GJ, Milione S, Kühl O, Hinrichs W, Pellecchia C
Inorg Chem 51 11220-2 (2012)
PMID: 23072298

Function and Biology Details

Reaction catalysed: 
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide deformylase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.24 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6K3 (Residues: 2-169; Coverage: 99%)
Gene names: JW3248, b3287, def, fms
Sequence domains: Polypeptide deformylase
Structure domains: Peptide deformylase

Ligands and Environments

3 bound ligands:
Ligand CO 1 x CO
Ligand CL 1 x CL
Ligand BME 1 x BME
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P6122
Unit cell:
a: 55.63Å b: 55.63Å c: 229.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Thiol reactive probes and chemosensors.
Peng et al. (2012)
3 more

1 mention without citation

Inhibition of Urease by Disulfiram, an FDA-Approved Thiol Reagent Used in Humans.
Díaz-Sánchez et al. (2016)