› 4aip

X-ray diffraction
2.4Å resolution

The FrpB iron transporter from Neisseria meningitidis (F3-3 variant)

Released:
DOI: 10.2210/pdb4aip/pdb
PDB entry 4aip coloured by chain, front view.
PDB entry 4aip coloured by chain, side view.
PDB entry 4aip coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Use of a molecular decoy to segregate transport from antigenicity in the FrpB iron transporter from Neisseria meningitidis.
Saleem M, Prince SM, Rigby SE, Imran M, Patel H, Chan H, Sanders H, Maiden MC, Feavers IM, Derrick JP
PLoS One 8 e56746 (2013)
PMID: 23457610

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175908 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe-regulated protein B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 742 amino acids
Theoretical weight: 82.32 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51162 (Residues: 23-720; Coverage: 100%)
Gene name: frpB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GLU 1 x GLU
Ligand N8E 2 x N8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P212121
Unit cell:
a: 85.32Å b: 104.62Å c: 269.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.242 0.296
Expression system: Escherichia coli

Quick links

Citations

2 review citations

How the Knowledge of Interactions between Meningococcus and the Human Immune System Has Been Used to Prepare Effective Neisseria meningitidis Vaccines.
Gasparini et al. (2015)
1 more

3 mentions without citation

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
2 more