› 4aaz

X-ray diffraction
1.4Å resolution

X-ray structure of Nicotiana alata Defensin 1 NaD1

Released:
DOI: 10.2210/pdb4aaz/pdb
PDB entry 4aaz coloured by chain, front view.
PDB entry 4aaz coloured by chain, side view.
PDB entry 4aaz coloured by chain, top view.
Source organism: Nicotiana alata
Primary publication:
Dimerization of plant defensin NaD1 enhances its antifungal activity.
Lay FT, Mills GD, Poon IK, Cowieson NP, Kirby N, Baxter AA, van der Weerden NL, Dogovski C, Perugini MA, Anderson MA, Kvansakul M, Hulett MD
J Biol Chem 287 19961-72 (2012)
PMID: 22511788

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flower-specific defensin Chains: A, B
Molecule details ›
Chains: A, B
Length: 47 amino acids
Theoretical weight: 5.32 KDa
Source organism: Nicotiana alata
UniProt:
  • Canonical: Q8GTM0 (Residues: 26-72; Coverage: 59%)
Gene name: D1
Sequence domains: Gamma-thionin family
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 32.697Å b: 32.685Å c: 41.977Å
α: 90° β: 100.83° γ: 90°
R-values:
R R work R free
0.122 0.121 0.137

Quick links

Citations

10 review citations

Antifungal plant defensins: mechanisms of action and production.
Vriens et al. (2014)
9 more

5 mentions without citation

Plant Defensins from a Structural Perspective.
Kovaleva et al. (2020)
4 more