› 4a3r

X-ray diffraction
2.2Å resolution

Crystal structure of Enolase from Bacillus subtilis.

Released:
DOI: 10.2210/pdb4a3r/pdb
PDB entry 4a3r coloured by chain, front view.
PDB entry 4a3r coloured by chain, side view.
PDB entry 4a3r coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Dissection of the network of interactions that links RNA processing with glycolysis in the Bacillus subtilis degradosome.
Newman JA, Hewitt L, Rodrigues C, Solovyova AS, Harwood CR, Lewis RJ
J Mol Biol 416 121-36 (2012)
PMID: 22198292

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 430 amino acids
Theoretical weight: 46.63 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P37869 (Residues: 1-430; Coverage: 100%)
Gene names: BSU33900, eno
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4
Unit cell:
a: 187.074Å b: 187.074Å c: 56.501Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.218
Expression system: Escherichia coli BL21

Quick links

Citations

12 review citations

RNA degradation in Bacillus subtilis: an interplay of essential endo- and exoribonucleases.
Lehnik-Habrink et al. (2012)
11 more

3 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
2 more